Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1

the OCCAMS consortium

doi:10.1038/srep32417

Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1

the OCCAMS consortium

University of Southampton
University of Manchester
Northern Care Alliance NHS Group
Wrightington, Wigan and Leigh NHS Trust
University of Cambridge
Cambridge University Hospitals NHS Foundation Trust
University of Oxford
Queen's University Belfast
Royal Surrey County Hospital NHS Foundation Trust
Edinburgh Royal Infirmary
University of Edinburgh
University Hospitals Birmingham NHS Foundation Trust
University of Birmingham
University Hospital Southampton NHS Foundation Trust
Gloucestershire Hospitals NHS Foundation Trust
St Sharrocks’s Hospital
King's College London
Karolinska Institutet
University Hospitals Plymouth NHS Trust
Norfolk and Norwich University Hospitals NHS Foundation Trust
Nottingham University Hospitals NHS Trust
University College London
Norfolk and Waveney Cellular Pathology Network
Manchester University NHS Foundation Trust
University Hospitals Coventry and Warwickshire NHS Trust
North West Anglia NHS Foundation Trust
University Hospitals of North Midlands NHS Trust
Cancer Research UK Cambridge Institute

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.

Original language	English
Article number	32417
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep32417
Publication status	Published - 7 Sept 2016
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1038/srep32417

Cite this

@article{5ac1185e510046a79f09a2dee246912d,

title = "Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1",

abstract = "New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.",

author = "\{the OCCAMS consortium\} and Edwin Garcia and Annette Hayden and Charles Birts and Edward Britton and Andrew Cowie and Karen Pickard and Massimiliano Mellone and Clarisa Choh and Mathieu Derouet and Patrick Duriez and Fergus Noble and White, \{Michael J.\} and Primrose, \{John N.\} and Strefford, \{Jonathan C.\} and Matthew Rose-Zerilli and Thomas, \{Gareth J.\} and Yeng Ang and Sharrocks, \{Andrew D.\} and Fitzgerald, \{Rebecca C.\} and Underwood, \{Timothy J.\} and Shona Macrae and Nicola Grehan and Zarah Abdullahi and \{de la Rue\}, Rachel and Ayesha Noorani and Elliott, \{Rachael Fels\} and \{de Silva\}, Nadeera and Jan Bornschein and Maria O{\textquoteright}donovan and Gianmarco Contino and Yang, \{Tsun Po\} and Hamza Chettouh and Jason Crawte and Barbara Nutzinger and Edwards, \{Paul A.W.\} and Laura Smith and Ahmad Miremadi and Shalini Malhotra and Alison Cluroe and Richard Hardwick and Jim Davies and Hugo Ford and David Gilligan and Peter Safranek and Andy Hindmarsh and Vijayendran Sujendran and Nick Carroll and Richard Turkington and Hayes, \{Stephen J.\} and Preston, \{Shaun R.\}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2016.",

year = "2016",

month = sep,

day = "7",

doi = "10.1038/srep32417",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

TY - JOUR

T1 - Authentication and characterisation of a new oesophageal adenocarcinoma cell line

T2 - MFD-1

AU - the OCCAMS consortium

AU - Garcia, Edwin

AU - Hayden, Annette

AU - Birts, Charles

AU - Britton, Edward

AU - Cowie, Andrew

AU - Pickard, Karen

AU - Mellone, Massimiliano

AU - Choh, Clarisa

AU - Derouet, Mathieu

AU - Duriez, Patrick

AU - Noble, Fergus

AU - White, Michael J.

AU - Primrose, John N.

AU - Strefford, Jonathan C.

AU - Rose-Zerilli, Matthew

AU - Thomas, Gareth J.

AU - Ang, Yeng

AU - Sharrocks, Andrew D.

AU - Fitzgerald, Rebecca C.

AU - Underwood, Timothy J.

AU - Macrae, Shona

AU - Grehan, Nicola

AU - Abdullahi, Zarah

AU - de la Rue, Rachel

AU - Noorani, Ayesha

AU - Elliott, Rachael Fels

AU - de Silva, Nadeera

AU - Bornschein, Jan

AU - O’donovan, Maria

AU - Contino, Gianmarco

AU - Yang, Tsun Po

AU - Chettouh, Hamza

AU - Crawte, Jason

AU - Nutzinger, Barbara

AU - Edwards, Paul A.W.

AU - Smith, Laura

AU - Miremadi, Ahmad

AU - Malhotra, Shalini

AU - Cluroe, Alison

AU - Hardwick, Richard

AU - Davies, Jim

AU - Ford, Hugo

AU - Gilligan, David

AU - Safranek, Peter

AU - Hindmarsh, Andy

AU - Sujendran, Vijayendran

AU - Carroll, Nick

AU - Turkington, Richard

AU - Hayes, Stephen J.

AU - Preston, Shaun R.

PY - 2016/9/7

Y1 - 2016/9/7

N2 - New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.

AB - New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.

UR - https://www.scopus.com/pages/publications/84987680020

U2 - 10.1038/srep32417

DO - 10.1038/srep32417

M3 - Article

C2 - 27600491

AN - SCOPUS:84987680020

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 32417

ER -

Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this