Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis

V. Parfejevs; M. Gavare; L. Cappiello; M. Grube; R. Muceniece; U. Riekstina

doi:10.3233/978-1-61499-184-7-221

Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis

V. Parfejevs
, M. Gavare
, L. Cappiello
, M. Grube
, R. Muceniece
, U. Riekstina^*

^*Corresponding author for this work

University of Latvia

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review

Abstract

Recently FT-IR analysis has been employed to study changes in molecular signatures during embryonic stem cell differentiation. We were interested to find out whether FT-IR spectroscopy could be applied to analyze changes in human skin mesenchymal stem cell (S-MSC) biochemical profile during in vitro neurodifferentiation. S-MSCs were propagated in serum-free medium with EGF and FGF-2 during six weeks. Neural progenitor cell line ReNcell CX (Millipore) was used as a reference cell line. Samples were collected each week and analyzed for neural marker nestin, tubulin βIII, GFAP and CD271 expression. FT-IR analysis was carried out using microplate reader HTS-XT (Bruker, Germany). Despite the immunophenotype similarity, FT-IR spectroscopy revealed distinct profiles for S-MSC culture and ReNcell CX cells. FT-IR spectra analyses showed changes of protein and lipid concentration during neurodifferentiation and different carbohydrate composition in ReNcell CX and S-MSCs. It was possible to discriminate between S-MSC cultures at different time-points during neurodifferentiation. The results of this study demonstrate that FT-IR spectroscopy is more sensitive than conventional immunophenotyping analysis and it has a great potential for the monitoring of the stem cell differentiation status.

Original language	English
Title of host publication	Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011
Editors	Maria Marques, Luis Batista de Carvalho, Parvez Haris
Pages	221-225
Number of pages	5
DOIs	https://doi.org/10.3233/978-1-61499-184-7-221
Publication status	Published - 2013

Publication series

Name	Advances in Biomedical Spectroscopy
Volume	7
ISSN (Print)	1875-0656

Keywords

FT-IR
neurodifferentiation
skin mesenchymal stem cells

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10.3233/978-1-61499-184-7-221

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Parfejevs, V., Gavare, M., Cappiello, L., Grube, M., Muceniece, R., & Riekstina, U. (2013). Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis. In M. Marques, L. Batista de Carvalho, & P. Haris (Eds.), Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011 (pp. 221-225). (Advances in Biomedical Spectroscopy; Vol. 7). https://doi.org/10.3233/978-1-61499-184-7-221

Parfejevs, V. ; Gavare, M. ; Cappiello, L. et al. / Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis. Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. editor / Maria Marques ; Luis Batista de Carvalho ; Parvez Haris. 2013. pp. 221-225 (Advances in Biomedical Spectroscopy).

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title = "Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis",

abstract = "Recently FT-IR analysis has been employed to study changes in molecular signatures during embryonic stem cell differentiation. We were interested to find out whether FT-IR spectroscopy could be applied to analyze changes in human skin mesenchymal stem cell (S-MSC) biochemical profile during in vitro neurodifferentiation. S-MSCs were propagated in serum-free medium with EGF and FGF-2 during six weeks. Neural progenitor cell line ReNcell CX (Millipore) was used as a reference cell line. Samples were collected each week and analyzed for neural marker nestin, tubulin βIII, GFAP and CD271 expression. FT-IR analysis was carried out using microplate reader HTS-XT (Bruker, Germany). Despite the immunophenotype similarity, FT-IR spectroscopy revealed distinct profiles for S-MSC culture and ReNcell CX cells. FT-IR spectra analyses showed changes of protein and lipid concentration during neurodifferentiation and different carbohydrate composition in ReNcell CX and S-MSCs. It was possible to discriminate between S-MSC cultures at different time-points during neurodifferentiation. The results of this study demonstrate that FT-IR spectroscopy is more sensitive than conventional immunophenotyping analysis and it has a great potential for the monitoring of the stem cell differentiation status.",

keywords = "FT-IR, neurodifferentiation, skin mesenchymal stem cells",

author = "V. Parfejevs and M. Gavare and L. Cappiello and M. Grube and R. Muceniece and U. Riekstina",

year = "2013",

doi = "10.3233/978-1-61499-184-7-221",

language = "English",

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Parfejevs, V, Gavare, M, Cappiello, L, Grube, M , Muceniece, R & Riekstina, U 2013, Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis. in M Marques, L Batista de Carvalho & P Haris (eds), Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. Advances in Biomedical Spectroscopy, vol. 7, pp. 221-225. https://doi.org/10.3233/978-1-61499-184-7-221

Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis. / Parfejevs, V.; Gavare, M.; Cappiello, L. et al.
Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. ed. / Maria Marques; Luis Batista de Carvalho; Parvez Haris. 2013. p. 221-225 (Advances in Biomedical Spectroscopy; Vol. 7).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review

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T1 - Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis

AU - Parfejevs, V.

AU - Gavare, M.

AU - Cappiello, L.

AU - Grube, M.

AU - Muceniece, R.

AU - Riekstina, U.

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N2 - Recently FT-IR analysis has been employed to study changes in molecular signatures during embryonic stem cell differentiation. We were interested to find out whether FT-IR spectroscopy could be applied to analyze changes in human skin mesenchymal stem cell (S-MSC) biochemical profile during in vitro neurodifferentiation. S-MSCs were propagated in serum-free medium with EGF and FGF-2 during six weeks. Neural progenitor cell line ReNcell CX (Millipore) was used as a reference cell line. Samples were collected each week and analyzed for neural marker nestin, tubulin βIII, GFAP and CD271 expression. FT-IR analysis was carried out using microplate reader HTS-XT (Bruker, Germany). Despite the immunophenotype similarity, FT-IR spectroscopy revealed distinct profiles for S-MSC culture and ReNcell CX cells. FT-IR spectra analyses showed changes of protein and lipid concentration during neurodifferentiation and different carbohydrate composition in ReNcell CX and S-MSCs. It was possible to discriminate between S-MSC cultures at different time-points during neurodifferentiation. The results of this study demonstrate that FT-IR spectroscopy is more sensitive than conventional immunophenotyping analysis and it has a great potential for the monitoring of the stem cell differentiation status.

AB - Recently FT-IR analysis has been employed to study changes in molecular signatures during embryonic stem cell differentiation. We were interested to find out whether FT-IR spectroscopy could be applied to analyze changes in human skin mesenchymal stem cell (S-MSC) biochemical profile during in vitro neurodifferentiation. S-MSCs were propagated in serum-free medium with EGF and FGF-2 during six weeks. Neural progenitor cell line ReNcell CX (Millipore) was used as a reference cell line. Samples were collected each week and analyzed for neural marker nestin, tubulin βIII, GFAP and CD271 expression. FT-IR analysis was carried out using microplate reader HTS-XT (Bruker, Germany). Despite the immunophenotype similarity, FT-IR spectroscopy revealed distinct profiles for S-MSC culture and ReNcell CX cells. FT-IR spectra analyses showed changes of protein and lipid concentration during neurodifferentiation and different carbohydrate composition in ReNcell CX and S-MSCs. It was possible to discriminate between S-MSC cultures at different time-points during neurodifferentiation. The results of this study demonstrate that FT-IR spectroscopy is more sensitive than conventional immunophenotyping analysis and it has a great potential for the monitoring of the stem cell differentiation status.

KW - FT-IR

KW - neurodifferentiation

KW - skin mesenchymal stem cells

UR - https://www.scopus.com/pages/publications/84888107906

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DO - 10.3233/978-1-61499-184-7-221

M3 - Chapter

AN - SCOPUS:84888107906

SN - 9781614991830

T3 - Advances in Biomedical Spectroscopy

SP - 221

EP - 225

BT - Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011

A2 - Marques, Maria

A2 - Batista de Carvalho, Luis

A2 - Haris, Parvez

ER -

Parfejevs V, Gavare M, Cappiello L, Grube M , Muceniece R , Riekstina U. Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis. In Marques M, Batista de Carvalho L, Haris P, editors, Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011. 2013. p. 221-225. (Advances in Biomedical Spectroscopy). doi: 10.3233/978-1-61499-184-7-221

Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis

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