@inproceedings{a0abab3599844addb2e5d4e677169fed,
title = "High volume extracellular vesicle separation in microfluidics: proof of principle",
abstract = "Extracellular vesicles (EV) have a potential to serve as novel disease biomarkers, however, currently EV isolation is complicated and with a high variability. Microfluidic devices utilizing multiple phenomena can be of use, however, to date reported devices cannot be used for high volume (>1 ml) samples. Herein, we show a proof of principle device based on asymmetric flow field-flow fractionation (A4F) and bifurcation-based microfluidic device leading to EV-sized bead enrichment of more than 200\% and more than 1000\% for cell-debrii sized beads. All while EV retention in these devices are more than 30\% lower than size exclusion column (SEC) experiments.",
keywords = "separation, asymmetric flow field-flow fractionation, Extracellular vesicles, microfluidics",
author = "Artūrs Ābols and Miks Priedols and Fēlikss Rūmnieks and Gunita Paidere and Gatis Mozoļevskis and Roberts Rim{\v s}a",
note = "Publisher Copyright: {\textcopyright} 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.",
year = "2022",
language = "English",
isbn = "978-173341904-8",
series = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
pages = "915--916",
booktitle = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}