Immunological localisation of melanocortin 1 receptor on the cell surface of WM266-4 human melanoma cells

Yun Xia; Ruta Muceniece; Jarl E.S. Wikberg

doi:10.1016/S0304-3835(06)80026-1

Immunological localisation of melanocortin 1 receptor on the cell surface of WM266-4 human melanoma cells

Yun Xia^*
, Ruta Muceniece
, Jarl E.S. Wikberg

^*Corresponding author for this work

Uppsala University
Latvian Institute of Organic Synthesis

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The localisation of melanocortin 1 receptor (MC1R) in WM266-4 human melanoma cells was investigated by applying an antipeptide antiserum specific for the cloned human MC1R (MSH receptor). In enzyme-linked immunosorbent assay (ELISA), the immunoreactivity was detected in the membrane fraction of WM266-4 cells. The ELISA reactivity could be inhibited by an antiserum pre-absorbed with its specific synthetic peptide. In immunocytochemistry, the specific immunoreactivity was demonstrated on the surface of the cells by using either biotin-avidin immunoalkaline phosphatase- or TRITC-staining method. These results indicate that the MC1R is prominently present on the plasma membrane of WM266-4 human melanoma cells.

Original language	English
Pages (from-to)	157-162
Number of pages	6
Journal	Cancer Letters
Volume	98
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3835(06)80026-1
Publication status	Published - 2 Jan 1996
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antibody
Melanocortin 1 receptor
Melanoma cell

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10.1016/S0304-3835(06)80026-1

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title = "Immunological localisation of melanocortin 1 receptor on the cell surface of WM266-4 human melanoma cells",

abstract = "The localisation of melanocortin 1 receptor (MC1R) in WM266-4 human melanoma cells was investigated by applying an antipeptide antiserum specific for the cloned human MC1R (MSH receptor). In enzyme-linked immunosorbent assay (ELISA), the immunoreactivity was detected in the membrane fraction of WM266-4 cells. The ELISA reactivity could be inhibited by an antiserum pre-absorbed with its specific synthetic peptide. In immunocytochemistry, the specific immunoreactivity was demonstrated on the surface of the cells by using either biotin-avidin immunoalkaline phosphatase- or TRITC-staining method. These results indicate that the MC1R is prominently present on the plasma membrane of WM266-4 human melanoma cells.",

keywords = "Antibody, Melanocortin 1 receptor, Melanoma cell",

author = "Yun Xia and Ruta Muceniece and Wikberg, \{Jarl E.S.\}",

year = "1996",

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doi = "10.1016/S0304-3835(06)80026-1",

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pages = "157--162",

journal = "Cancer Letters",

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T1 - Immunological localisation of melanocortin 1 receptor on the cell surface of WM266-4 human melanoma cells

AU - Xia, Yun

AU - Muceniece, Ruta

AU - Wikberg, Jarl E.S.

PY - 1996/1/2

Y1 - 1996/1/2

N2 - The localisation of melanocortin 1 receptor (MC1R) in WM266-4 human melanoma cells was investigated by applying an antipeptide antiserum specific for the cloned human MC1R (MSH receptor). In enzyme-linked immunosorbent assay (ELISA), the immunoreactivity was detected in the membrane fraction of WM266-4 cells. The ELISA reactivity could be inhibited by an antiserum pre-absorbed with its specific synthetic peptide. In immunocytochemistry, the specific immunoreactivity was demonstrated on the surface of the cells by using either biotin-avidin immunoalkaline phosphatase- or TRITC-staining method. These results indicate that the MC1R is prominently present on the plasma membrane of WM266-4 human melanoma cells.

AB - The localisation of melanocortin 1 receptor (MC1R) in WM266-4 human melanoma cells was investigated by applying an antipeptide antiserum specific for the cloned human MC1R (MSH receptor). In enzyme-linked immunosorbent assay (ELISA), the immunoreactivity was detected in the membrane fraction of WM266-4 cells. The ELISA reactivity could be inhibited by an antiserum pre-absorbed with its specific synthetic peptide. In immunocytochemistry, the specific immunoreactivity was demonstrated on the surface of the cells by using either biotin-avidin immunoalkaline phosphatase- or TRITC-staining method. These results indicate that the MC1R is prominently present on the plasma membrane of WM266-4 human melanoma cells.

KW - Antibody

KW - Melanocortin 1 receptor

KW - Melanoma cell

UR - https://www.scopus.com/pages/publications/0030063467

U2 - 10.1016/S0304-3835(06)80026-1

DO - 10.1016/S0304-3835(06)80026-1

M3 - Article

C2 - 8556703

AN - SCOPUS:0030063467

SN - 0304-3835

VL - 98

SP - 157

EP - 162

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Immunological localisation of melanocortin 1 receptor on the cell surface of WM266-4 human melanoma cells

Abstract

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Keywords

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