Mosaic Qβ coats as a new presentation model

Inta Vasiljeva; Tatjana Kozlovska; Indulis Cielens; Anna Strelnikova; Andris Kazaks; Velta Ose; Paul Pumpens

doi:10.1016/S0014-5793(98)00716-9

Mosaic Qβ coats as a new presentation model

Inta Vasiljeva
, Tatjana Kozlovska
, Indulis Cielens
, Anna Strelnikova
, Andris Kazaks
, Velta Ose
, Paul Pumpens^*

^*Corresponding author for this work

The Faculty of Medicine and Life Sciences

University of Latvia

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

The new protein carrier was developed on the basis of recombinant RNA phage Qβ capsid. C-terminal UGA extension of the short form of Qβ coat, so-called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qβ particles. In conditions of enhanced UGA suppression, the proportion of A1-extended to short coats in mosaic particles dropped from 48% to 14%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31=DPAFR-35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qβ particles and ensured specific antigenicity and immunogenicity.

Original language	English
Pages (from-to)	7-11
Number of pages	5
Journal	FEBS Letters
Volume	431
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(98)00716-9
Publication status	Published - 10 Jul 1998

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

A1 extension
Capsid assembly
Coat protein UGA suppression
Hepatitis B virus preS1
Immunogenicity
Phage Qβ

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10.1016/S0014-5793(98)00716-9

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abstract = "The new protein carrier was developed on the basis of recombinant RNA phage Qβ capsid. C-terminal UGA extension of the short form of Qβ coat, so-called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qβ particles. In conditions of enhanced UGA suppression, the proportion of A1-extended to short coats in mosaic particles dropped from 48\% to 14\%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31=DPAFR-35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qβ particles and ensured specific antigenicity and immunogenicity.",

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T1 - Mosaic Qβ coats as a new presentation model

AU - Vasiljeva, Inta

AU - Kozlovska, Tatjana

AU - Cielens, Indulis

AU - Strelnikova, Anna

AU - Kazaks, Andris

AU - Ose, Velta

AU - Pumpens, Paul

PY - 1998/7/10

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KW - Capsid assembly

KW - Coat protein UGA suppression

KW - Hepatitis B virus preS1

KW - Immunogenicity

KW - Phage Qβ

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Mosaic Qβ coats as a new presentation model

Abstract

UN SDGs

Keywords

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