Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital

Uga Dumpis; Aina Iversen; Arta Balode; Mara Saule; Edvins Miklaševičs; Christian G. Giske

doi:10.1111/j.1600-0463.2010.02640.x

Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital

Uga Dumpis
, Aina Iversen
, Arta Balode
, Mara Saule
, Edvins Miklaševičs
, Christian G. Giske

Paula Stradina Clinical University Hospital
Karolinska Institutet
University of Latvia
Riga Stradins University

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Previous studies on the epidemiology of extended-spectrum -lactamase (ESBL)-producing Enterobacteriaceae in Latvia are lacking. ESBL-producing Klebsiella pneumoniae (n = 32) were subjected to pulsed-field gel electrophoresis (PFGE) and selected isolates to multi-locus sequence typing (MLST). Species identification and susceptibility testing were performed using VITEK2, and sequencing of bla_CTX-M was performed in selected isolates. PFGE revealed one major clone (n = 23), with most of the isolates derived from the ICU. The clone harboured bla_CTX-M-15, was sequence type 199 and comprised two ertapenem non-susceptible isolates. This is the first report of an ESBL outbreak in Latvia, and calls for increased epidemiological typing of ESBL-producing Enterobacteriaceae, as well as improved infection control routines.

Original language	English
Pages (from-to)	713-716
Number of pages	4
Journal	APMIS
Volume	118
Issue number	9
DOIs	https://doi.org/10.1111/j.1600-0463.2010.02640.x
Publication status	Published - Sept 2010

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

clonal complex
CTX-M
ESBL
MLST
ST

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10.1111/j.1600-0463.2010.02640.x

Cite this

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title = "Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital",

abstract = "Previous studies on the epidemiology of extended-spectrum -lactamase (ESBL)-producing Enterobacteriaceae in Latvia are lacking. ESBL-producing Klebsiella pneumoniae (n = 32) were subjected to pulsed-field gel electrophoresis (PFGE) and selected isolates to multi-locus sequence typing (MLST). Species identification and susceptibility testing were performed using VITEK2, and sequencing of blaCTX-M was performed in selected isolates. PFGE revealed one major clone (n = 23), with most of the isolates derived from the ICU. The clone harboured blaCTX-M-15, was sequence type 199 and comprised two ertapenem non-susceptible isolates. This is the first report of an ESBL outbreak in Latvia, and calls for increased epidemiological typing of ESBL-producing Enterobacteriaceae, as well as improved infection control routines.",

keywords = "clonal complex, CTX-M, ESBL, MLST, ST",

author = "Uga Dumpis and Aina Iversen and Arta Balode and Mara Saule and Edvins Mikla{\v s}evi{\v c}s and Giske, \{Christian G.\}",

year = "2010",

month = sep,

doi = "10.1111/j.1600-0463.2010.02640.x",

language = "English",

volume = "118",

pages = "713--716",

journal = "APMIS",

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T1 - Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital

AU - Dumpis, Uga

AU - Iversen, Aina

AU - Balode, Arta

AU - Saule, Mara

AU - Miklaševičs, Edvins

AU - Giske, Christian G.

PY - 2010/9

Y1 - 2010/9

N2 - Previous studies on the epidemiology of extended-spectrum -lactamase (ESBL)-producing Enterobacteriaceae in Latvia are lacking. ESBL-producing Klebsiella pneumoniae (n = 32) were subjected to pulsed-field gel electrophoresis (PFGE) and selected isolates to multi-locus sequence typing (MLST). Species identification and susceptibility testing were performed using VITEK2, and sequencing of blaCTX-M was performed in selected isolates. PFGE revealed one major clone (n = 23), with most of the isolates derived from the ICU. The clone harboured blaCTX-M-15, was sequence type 199 and comprised two ertapenem non-susceptible isolates. This is the first report of an ESBL outbreak in Latvia, and calls for increased epidemiological typing of ESBL-producing Enterobacteriaceae, as well as improved infection control routines.

AB - Previous studies on the epidemiology of extended-spectrum -lactamase (ESBL)-producing Enterobacteriaceae in Latvia are lacking. ESBL-producing Klebsiella pneumoniae (n = 32) were subjected to pulsed-field gel electrophoresis (PFGE) and selected isolates to multi-locus sequence typing (MLST). Species identification and susceptibility testing were performed using VITEK2, and sequencing of blaCTX-M was performed in selected isolates. PFGE revealed one major clone (n = 23), with most of the isolates derived from the ICU. The clone harboured blaCTX-M-15, was sequence type 199 and comprised two ertapenem non-susceptible isolates. This is the first report of an ESBL outbreak in Latvia, and calls for increased epidemiological typing of ESBL-producing Enterobacteriaceae, as well as improved infection control routines.

KW - clonal complex

KW - CTX-M

KW - ESBL

KW - MLST

KW - ST

UR - https://www.scopus.com/pages/publications/77955948005

U2 - 10.1111/j.1600-0463.2010.02640.x

DO - 10.1111/j.1600-0463.2010.02640.x

M3 - Article

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AN - SCOPUS:77955948005

SN - 0903-4641

VL - 118

SP - 713

EP - 716

JO - APMIS

JF - APMIS

IS - 9

ER -

Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital

Abstract

UN SDGs

Keywords

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