Oxidative phosphorylation in Zymomonas mobilis

U. Kalnenieks; A. A. de Graaf; S. Bringer-Meyer; H. Sahm

doi:10.1007/BF00258148

Oxidative phosphorylation in Zymomonas mobilis

U. Kalnenieks^*
, A. A. de Graaf
, S. Bringer-Meyer
, H. Sahm

^*Corresponding author for this work

Jülich Research Centre

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

The obligately fermentative aerotolerant bacterium Zymomonas mobilis was shown to possess oxidative phosphorylation activity. Increased intracellular ATP levels were observed in aerated starved cell suspension in the presence of ethanol or acetaldehyde. Ethanolconsuming Z. mobilis generated a transmembrane pH gradient. ATP synthesis in starved Z. mobilis cells could be induced by external medium acidification of 3.5-4.0 pH units. Membrane vesicles of Z. mobilis coupled ATP synthesis to NADH oxidation. ATP synthesis was sensitive to the protonophoric uncoupler CCCP both in starved cells and in membrane vesicles. The H⁺-ATPase inhibitor DCCD was shown to inhibit the NADH-coupled ATP synthesis in membrane vesicles. The physiological role of oxidative phosphorylation in this obligately fermentative bacterium is discussed.

Original language	English
Pages (from-to)	74-79
Number of pages	6
Journal	Archives of Microbiology
Volume	160
Issue number	1
DOIs	https://doi.org/10.1007/BF00258148
Publication status	Published - Jul 1993
Externally published	Yes

Keywords

P-NMR
Acetaldehyde
ATP synthesis
Ethanol metabolism
membrane vesicles
Oxidative phosphorylation
transmembrane pH gradient
Zymomonas mobilis

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10.1007/BF00258148

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title = "Oxidative phosphorylation in Zymomonas mobilis",

abstract = "The obligately fermentative aerotolerant bacterium Zymomonas mobilis was shown to possess oxidative phosphorylation activity. Increased intracellular ATP levels were observed in aerated starved cell suspension in the presence of ethanol or acetaldehyde. Ethanolconsuming Z. mobilis generated a transmembrane pH gradient. ATP synthesis in starved Z. mobilis cells could be induced by external medium acidification of 3.5-4.0 pH units. Membrane vesicles of Z. mobilis coupled ATP synthesis to NADH oxidation. ATP synthesis was sensitive to the protonophoric uncoupler CCCP both in starved cells and in membrane vesicles. The H+-ATPase inhibitor DCCD was shown to inhibit the NADH-coupled ATP synthesis in membrane vesicles. The physiological role of oxidative phosphorylation in this obligately fermentative bacterium is discussed.",

keywords = "P-NMR, Acetaldehyde, ATP synthesis, Ethanol metabolism, membrane vesicles, Oxidative phosphorylation, transmembrane pH gradient, Zymomonas mobilis",

author = "U. Kalnenieks and \{de Graaf\}, \{A. A.\} and S. Bringer-Meyer and H. Sahm",

year = "1993",

month = jul,

doi = "10.1007/BF00258148",

language = "English",

volume = "160",

pages = "74--79",

journal = "Archives of Microbiology",

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TY - JOUR

T1 - Oxidative phosphorylation in Zymomonas mobilis

AU - Kalnenieks, U.

AU - de Graaf, A. A.

AU - Bringer-Meyer, S.

AU - Sahm, H.

PY - 1993/7

Y1 - 1993/7

N2 - The obligately fermentative aerotolerant bacterium Zymomonas mobilis was shown to possess oxidative phosphorylation activity. Increased intracellular ATP levels were observed in aerated starved cell suspension in the presence of ethanol or acetaldehyde. Ethanolconsuming Z. mobilis generated a transmembrane pH gradient. ATP synthesis in starved Z. mobilis cells could be induced by external medium acidification of 3.5-4.0 pH units. Membrane vesicles of Z. mobilis coupled ATP synthesis to NADH oxidation. ATP synthesis was sensitive to the protonophoric uncoupler CCCP both in starved cells and in membrane vesicles. The H+-ATPase inhibitor DCCD was shown to inhibit the NADH-coupled ATP synthesis in membrane vesicles. The physiological role of oxidative phosphorylation in this obligately fermentative bacterium is discussed.

AB - The obligately fermentative aerotolerant bacterium Zymomonas mobilis was shown to possess oxidative phosphorylation activity. Increased intracellular ATP levels were observed in aerated starved cell suspension in the presence of ethanol or acetaldehyde. Ethanolconsuming Z. mobilis generated a transmembrane pH gradient. ATP synthesis in starved Z. mobilis cells could be induced by external medium acidification of 3.5-4.0 pH units. Membrane vesicles of Z. mobilis coupled ATP synthesis to NADH oxidation. ATP synthesis was sensitive to the protonophoric uncoupler CCCP both in starved cells and in membrane vesicles. The H+-ATPase inhibitor DCCD was shown to inhibit the NADH-coupled ATP synthesis in membrane vesicles. The physiological role of oxidative phosphorylation in this obligately fermentative bacterium is discussed.

KW - P-NMR

KW - Acetaldehyde

KW - ATP synthesis

KW - Ethanol metabolism

KW - membrane vesicles

KW - Oxidative phosphorylation

KW - transmembrane pH gradient

KW - Zymomonas mobilis

UR - https://www.scopus.com/pages/publications/0027211769

U2 - 10.1007/BF00258148

DO - 10.1007/BF00258148

M3 - Article

AN - SCOPUS:0027211769

SN - 0302-8933

VL - 160

SP - 74

EP - 79

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 1

ER -

Oxidative phosphorylation in Zymomonas mobilis

Abstract

Keywords

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