Paņēmiens tiopurīna metiltransferāzes polimorfizmu noteikšanai.

The invention relates to methods and substances for the detection of thiopurine methyltransferase polymorphisms (TPMT). The method for the detection of TPMT polymorphisms comprises the following sequential steps: (i) providing a biological sample containing patient DNA; (ii) providing a first mixture containing primers and two hydrolysis probes adapted for the amplification of the DNA target sequence and the detection of the TPMT gene polymorphism rs1800462 (TPMT*2); (iii) providing a second mixture containing primers and two hydrolysis probes adapted for the amplification of the DNA target sequence and the detection of the TPMT gene polymorphism rs1800460 (TPMT*3B); (iv) providing a third mixture containing primers and two hydrolysis probes adapted for the amplification of the DNA target sequence and the detection of the TPMT gene polymorphism rs1142345 (TPMT*3C); (v) providing a polymerase chain reaction (PCR) premix; (vi) adding DNA extracted from the biological sample and the premix to the first, second and third mixtures for performing quantitative PCR and amplifying the target nucleic acid; (vii) measuring fluorescence and determining TPMT gene polymorphisms by comparing the amplification results with previously known samples.