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Porous silicon photoluminescence biosensor for rapid and sensitive detection of toxins

  • Yulia Melnyk
  • , Karyna Pavlova
  • , Valerii Myndrul
  • , Roman Viter
  • , Valentyn Smyntyna
  • , Igor Iatsunskyi
  • Odessa National University
  • Adam Mickiewicz University in Poznań

Research output: Chapter in Book/Report/Conference proceedingConference paperResearchpeer-review

1 Citation (Scopus)

Abstract

A rapid and low cost photoluminescence (PL) immunosensor for the determination of low concentrations of Ochratoxin A(OTA) and Aflatoxine B1 (AfB1) has been developed. This biosensor was based on porous silicon (PSi) fabricated by metal-assisted chemical etching (MACE) and modified by antibodies against OTA/AfB1 (anti-OTA/anti-AfB1). Biofunctionalization method of the PSi surface by anti-OTA/anti-AfB1 was developed. The changes of the PL intensity after interaction of the immobilized anti-OTA/anti-AfB1with OTA/AfB1 antigens were used as biosensor signal, allowing sensitive and selective detection of OTA/AfB1 antigens in BSA solution. The sensitivity of the reported optical biosensor towards OTA/AfB1 antigens is in the range from 10-3 to 102 ng/ml.

Original languageEnglish
Title of host publicationOrganic Sensors and Bioelectronics X
EditorsIoannis Kymissis, Luisa Torsi, Ruth Shinar
PublisherSPIE
ISBN (Electronic)9781510611856
DOIs
Publication statusPublished - 2017
EventOrganic Sensors and Bioelectronics X 2017 - San Diego, United States
Duration: 6 Aug 20177 Aug 2017

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10364
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceOrganic Sensors and Bioelectronics X 2017
Country/TerritoryUnited States
CitySan Diego
Period6/08/177/08/17

Keywords

  • Aflatoxine B1
  • Biofunctionalization.
  • Biosensor
  • Ochratoxin A
  • Porous Silicon

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