Protein and solvent dynamics of the water-soluble chlorophyll-binding protein (WSCP)

Leonid Rusevich; Jan Embs; Inga Bektas; Harald Paulsen; Gernot Renger; Jörg Pieper

doi:10.1051/epjconf/20158302016

Protein and solvent dynamics of the water-soluble chlorophyll-binding protein (WSCP)

Leonid Rusevich
, Jan Embs
, Inga Bektas
, Harald Paulsen
, Gernot Renger
, Jörg Pieper^*

^*Corresponding author for this work

Institute of Physical Energetics
Paul Scherrer Institute
Johannes Gutenberg University Mainz
Technical University of Berlin
University of Tartu

Research output: Contribution to journal › Conference article › peer-review

6 Citations (Scopus)

Abstract

This study presents quasielastic neutron scattering data of the water-soluble chlorophyll-binding protein (WSCP) and the corresponding buffer solution at room temperature. The contributions of protein and buffer solution to the overall scattering are carefully separated. Otherwise, the fast water dynamics dominating the buffer contribution is likely to mask the slow protein dynamics. In the case of WSCP, the protein scattering can be described by two contributions: i) internal protein dynamics represented by a diffusion in a sphere with an average radius of 2.7 Å and ii) global (Brownian) diffusion of the WSCP macromolecule with an upper limit for the translational diffusion coefficient of 9.4 · 10^-7cm²/s.

Original language	English
Article number	02016
Journal	EPJ Web of Conferences
Volume	83
DOIs	https://doi.org/10.1051/epjconf/20158302016
Publication status	Published - 23 Jan 2015
Externally published	Yes
Event	11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014 - Autrans, France Duration: 11 May 2014 → 16 May 2014

Access to Document

10.1051/epjconf/20158302016

Cite this

@article{7b05450d347a40078d8f3ef9a7229c9f,

title = "Protein and solvent dynamics of the water-soluble chlorophyll-binding protein (WSCP)",

abstract = "This study presents quasielastic neutron scattering data of the water-soluble chlorophyll-binding protein (WSCP) and the corresponding buffer solution at room temperature. The contributions of protein and buffer solution to the overall scattering are carefully separated. Otherwise, the fast water dynamics dominating the buffer contribution is likely to mask the slow protein dynamics. In the case of WSCP, the protein scattering can be described by two contributions: i) internal protein dynamics represented by a diffusion in a sphere with an average radius of 2.7 {\AA} and ii) global (Brownian) diffusion of the WSCP macromolecule with an upper limit for the translational diffusion coefficient of 9.4 · 10-7cm2/s.",

author = "Leonid Rusevich and Jan Embs and Inga Bektas and Harald Paulsen and Gernot Renger and J{\"o}rg Pieper",

note = "Publisher Copyright: {\textcopyright} 2015 Owned by the authors, published by EDP Sciences.; 11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014 ; Conference date: 11-05-2014 Through 16-05-2014",

year = "2015",

month = jan,

day = "23",

doi = "10.1051/epjconf/20158302016",

language = "English",

volume = "83",

journal = "EPJ Web of Conferences",

issn = "2101-6275",

publisher = "EDP Sciences",

}

TY - JOUR

T1 - Protein and solvent dynamics of the water-soluble chlorophyll-binding protein (WSCP)

AU - Rusevich, Leonid

AU - Embs, Jan

AU - Bektas, Inga

AU - Paulsen, Harald

AU - Renger, Gernot

AU - Pieper, Jörg

PY - 2015/1/23

Y1 - 2015/1/23

N2 - This study presents quasielastic neutron scattering data of the water-soluble chlorophyll-binding protein (WSCP) and the corresponding buffer solution at room temperature. The contributions of protein and buffer solution to the overall scattering are carefully separated. Otherwise, the fast water dynamics dominating the buffer contribution is likely to mask the slow protein dynamics. In the case of WSCP, the protein scattering can be described by two contributions: i) internal protein dynamics represented by a diffusion in a sphere with an average radius of 2.7 Å and ii) global (Brownian) diffusion of the WSCP macromolecule with an upper limit for the translational diffusion coefficient of 9.4 · 10-7cm2/s.

AB - This study presents quasielastic neutron scattering data of the water-soluble chlorophyll-binding protein (WSCP) and the corresponding buffer solution at room temperature. The contributions of protein and buffer solution to the overall scattering are carefully separated. Otherwise, the fast water dynamics dominating the buffer contribution is likely to mask the slow protein dynamics. In the case of WSCP, the protein scattering can be described by two contributions: i) internal protein dynamics represented by a diffusion in a sphere with an average radius of 2.7 Å and ii) global (Brownian) diffusion of the WSCP macromolecule with an upper limit for the translational diffusion coefficient of 9.4 · 10-7cm2/s.

UR - https://www.scopus.com/pages/publications/84921720610

U2 - 10.1051/epjconf/20158302016

DO - 10.1051/epjconf/20158302016

M3 - Conference article

AN - SCOPUS:84921720610

SN - 2101-6275

VL - 83

JO - EPJ Web of Conferences

JF - EPJ Web of Conferences

M1 - 02016

T2 - 11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014

Y2 - 11 May 2014 through 16 May 2014

ER -

Protein and solvent dynamics of the water-soluble chlorophyll-binding protein (WSCP)

Abstract

Access to Document

Other files and links

Fingerprint

Cite this