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Single-feature polymorphism discovery in the barley transcriptome.

  • Nils Rostoks*
  • , Justin O. Borevitz
  • , Peter E. Hedley
  • , Joanne Russell
  • , Sharon Mudie
  • , Jenny Morris
  • , Linda Cardle
  • , David F. Marshall
  • , Robbie Waugh
  • *Corresponding author for this work
  • The James Hutton Institute

Research output: Contribution to journalArticlepeer-review

136 Citations (Scopus)

Abstract

A probe-level model for analysis of GeneChip gene-expression data is presented which identified more than 10,000 single-feature polymorphisms (SFP) between two barley genotypes. The method has good sensitivity, as 67% of known single-nucleotide polymorphisms (SNP) were called as SFPs. This method is applicable to all oligonucleotide microarray data, accounts for SNP effects in gene-expression data and represents an efficient and versatile approach for highly parallel marker identification in large genomes.

Original languageEnglish
Pages (from-to)R54
JournalGenome Biology
Volume6
Issue number6
Publication statusPublished - 2005
Externally publishedYes

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