Soil and tree stem xylem water isotope data from two pan-European sampling campaigns

The stable isotope ratios of hydrogen (δ2H) and oxygen (δ18O) are useful for studying ecohydrological dynamics in forests. However, most isotope-based eco-hydrological studies are limited to single sites, resulting in a lack of large-scale isotope data for understanding tree water uptake. Here, we provide a first systematic isotope dataset for soil and stem xylem water collected during two pan-European sampling campaigns at 40 beech (Fagus sylvatica), spruce (Picea abies), or mixed beech-spruce forest sites in spring and summer 2023 (10.16904/envidat.542, Lehmann et al., 2024). The dataset is complemented by additional site-, soil-, and tree-specific metadata. The samples and metadata were collected by different researchers across Europe following a standardized protocol. Soil samples were taken at up to 5 depths (ranging from 0 to 90 cm) and stem xylem samples from the trunks of three beech and/or spruce trees per site. All samples were sent to a single laboratory, where all analytical work was conducted. Water was extracted using cryogenic vacuum distillation and analyzed with an isotope laser spectrometer. Additionally, a subset of the samples was analyzed with an isotope ratio mass spectrometer. Data quality checks revealed a high mean total extraction efficiency, mean water amount (>1mL), accuracy, and precision. The isotopic signature of soil and stem xylem water varied as a function of the geographic origin and changed from spring to summer across all sites. While δ2H and δ18O were strongly correlated, the soil water data plotted closer to the Global Meteoric Water Line (GMWL) than the stem xylem water. Specifically, the δ2H values of the xylem water were more enriched than those of the soil water, leading to a systematic deviation from the GMWL. Isotopic enrichment of the stem xylem water at mixed forest sites was larger for spruce trees than for beech trees. This dataset is particularly useful for large-scale studies on plant water use, ecohydrological model testing, and isotope mapping across Europe.