Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

Solveiga Grinberga; Maija Dambrova; Gustavs Latkovskis; Ieva Strele; Ilze Konrade; Dace Hartmane; Eduards Sevostjanovs; Edgars Liepinsh; Osvalds Pugovics

doi:10.1002/bmc.3477

Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

Solveiga Grinberga^*
, Maija Dambrova
, Gustavs Latkovskis
, Ieva Strele
, Ilze Konrade
, Dace Hartmane
, Eduards Sevostjanovs
, Edgars Liepinsh
, Osvalds Pugovics

^*Šī darba korespondējošais autors

Latvian Institute of Organic Synthesis
Riga Stradins University
Paula Stradina Clinical University Hospital

Zinātniskās darbības rezultāts: Devums žurnālam › Zinātniskais raksts (žurnālā) › koleģiāli recenzēts

36 Atsauces (Scopus)

Kopsavilkums

An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.

Oriģinālvaloda	Angļu
Lapas (no-līdz)	1670-1674
Lapu skaits	5
Žurnāls	Biomedical Chromatography
Sējums	29
Izdevuma numurs	11
DOIs	https://doi.org/10.1002/bmc.3477
Publikācijas statuss	Publicēts - nov. 2015

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title = "Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS",

abstract = "An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.",

keywords = "Human plasma, L-carnitine, Trimethylamine N-oxide, UPLC/MS/MS, γ-butyrobetaine",

author = "Solveiga Grinberga and Maija Dambrova and Gustavs Latkovskis and Ieva Strele and Ilze Konrade and Dace Hartmane and Eduards Sevostjanovs and Edgars Liepinsh and Osvalds Pugovics",

note = "Publisher Copyright: {\textcopyright} 2015 John Wiley \& Sons, Ltd.",

year = "2015",

month = nov,

doi = "10.1002/bmc.3477",

language = "English",

volume = "29",

pages = "1670--1674",

journal = "Biomedical Chromatography",

issn = "0269-3879",

publisher = "John Wiley and Sons Ltd",

number = "11",

}

Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS. / Grinberga, Solveiga; Dambrova, Maija; Latkovskis, Gustavs u.c.
(gadā): Biomedical Chromatography, Sēj. 29, Nr. 11, 11.2015, lpp. 1670-1674.

Zinātniskās darbības rezultāts: Devums žurnālam › Zinātniskais raksts (žurnālā) › koleģiāli recenzēts

TY - JOUR

T1 - Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

AU - Grinberga, Solveiga

AU - Dambrova, Maija

AU - Latkovskis, Gustavs

AU - Strele, Ieva

AU - Konrade, Ilze

AU - Hartmane, Dace

AU - Sevostjanovs, Eduards

AU - Liepinsh, Edgars

AU - Pugovics, Osvalds

PY - 2015/11

Y1 - 2015/11

N2 - An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.

AB - An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules l-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.

KW - Human plasma

KW - L-carnitine

KW - Trimethylamine N-oxide

KW - UPLC/MS/MS

KW - γ-butyrobetaine

UR - https://www.scopus.com/pages/publications/84943659696

U2 - 10.1002/bmc.3477

DO - 10.1002/bmc.3477

M3 - Article

C2 - 25873316

AN - SCOPUS:84943659696

SN - 0269-3879

VL - 29

SP - 1670

EP - 1674

JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 11

ER -

Determination of trimethylamine-N-oxide in combination with l-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS

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