Diel variation in CO2 flux is substantial in many lakes: Diel variation in CO₂ flux is substantial in many lakes

Lakes play a significant role in the global carbon cycle, acting as sources and sinks of carbon dioxide (CO₂). In situ measurements of CO₂ flux (FCO₂) from lakes have generally been collected during daylight, despite indications of significant diel variability. This introduces bias when scaling up to whole-lake annual aquatic carbon budgets. We conducted an international sampling program to ascertain the extent of diel variation in FCO₂ across lakes. We sampled 21 lakes over 41 campaigns and measured FCO₂ at 4-h intervals over a full diel cycle. Rates of FCO₂ ranged from −3.16 to 4.39 mmol m⁻² h⁻¹. Integrated over a day, FCO₂ ranged from −381.68 to 878.49 mg C m⁻² d⁻¹ (mean = 76.54) across campaigns. We identified three characteristic diel patterns in FCO₂ related to trophic status and show that for half of the campaigns, daily flux estimates were biased by > 50% if based on a single (daytime) measurement.