@inbook{58e22d2e01ff4192a9d7e04464f00213,
title = "Evaluation of biochemical changes in skin-derived mesenchymal stem cells during in vitro neurodifferentiation by FT-IR analysis",
abstract = "Recently FT-IR analysis has been employed to study changes in molecular signatures during embryonic stem cell differentiation. We were interested to find out whether FT-IR spectroscopy could be applied to analyze changes in human skin mesenchymal stem cell (S-MSC) biochemical profile during in vitro neurodifferentiation. S-MSCs were propagated in serum-free medium with EGF and FGF-2 during six weeks. Neural progenitor cell line ReNcell CX (Millipore) was used as a reference cell line. Samples were collected each week and analyzed for neural marker nestin, tubulin βIII, GFAP and CD271 expression. FT-IR analysis was carried out using microplate reader HTS-XT (Bruker, Germany). Despite the immunophenotype similarity, FT-IR spectroscopy revealed distinct profiles for S-MSC culture and ReNcell CX cells. FT-IR spectra analyses showed changes of protein and lipid concentration during neurodifferentiation and different carbohydrate composition in ReNcell CX and S-MSCs. It was possible to discriminate between S-MSC cultures at different time-points during neurodifferentiation. The results of this study demonstrate that FT-IR spectroscopy is more sensitive than conventional immunophenotyping analysis and it has a great potential for the monitoring of the stem cell differentiation status.",
keywords = "FT-IR, neurodifferentiation, skin mesenchymal stem cells",
author = "V. Parfejevs and M. Gavare and L. Cappiello and M. Grube and R. Muceniece and U. Riekstina",
year = "2013",
doi = "10.3233/978-1-61499-184-7-221",
language = "English",
isbn = "9781614991830",
series = "Advances in Biomedical Spectroscopy",
pages = "221--225",
editor = "Maria Marques and \{Batista de Carvalho\}, Luis and Parvez Haris",
booktitle = "Spectroscopy of Biological Molecules. Proceedings from the 14th European Conference on the Spectroscopy of Biological Molecules 2011",
}