Optical anisotropy of cell nuclei

Optical anisotropy in cell nuclei are associated with a subcellular organisation of nucleus. The differences in a breast adenocarcinomas toluidine blue stained chromatin birefringence intensity indicate differences in the chromatin Stereoarrangement in cell nuclei. In unstained paraffin sections origin of cell nuclei birefringence could be due to nonhistone proteins which appropriate conformational state developed during the paraffin embedding procedure. The unstained paraffin section cell nuclei birefringence could be useful optical feature for survival prognosis of patients with cancer.