Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Bovine leukaemia virus (BLV) proteins gp51, which are serving as antigens for specific antibodies against BLV proteins (anti-gp51), were applied as biological recognition part in the design of immunosensor devoted for the determination of anti-gp51. The efficiency of the immobilization of BLV proteins gp51 on ZnO nanorod (ZnO-NR) modified glass (ZnO-NR/glass) surface was evaluated. The formation of antigen-antibody complex on the ZnO/glass modified by the BLV proteins gp51 (gp51/ZnO-NR/glass) was investigated by the determination of changes in ZnO photoluminescence. The applicability of gp51/ZnO-NR/glass in the design of photoluminescence based immunosensor was evaluated. Bovine serum albumin (BSA) was applied for the modification of sensing gp51 layer in order to form gp51&BSA layer with advanced selectivity. Polyallylamine hydrochloride (PAH) was applied in order to improve the immobilization of gp51 and BSA based sensing layer (gp51&BSA) on the surface of ZnO-NR/glass. PAH was applied during the formation of gp51&BSA/PAH/ZnO-NR/glass structure. Some aspects of the mechanism of interaction between biomolecules (gp51, BSA and anti-gp51) and ZnO-NR during the preparation and action of gp51&BSA/ZnO-NR/glass- and gp51&BSA/PAH/ZnO-NR/glass-based immunosensors have been discussed.